| 2009-04-25 | HullvLiverpoolPremier League · England | 1–3 | Premier League · England |
| 2009-04-25 | Queen of SthvClydeChampionship · Scotland | 7–1 | Championship · Scotland |
| 2009-04-25 | SwindonvBristol RvsLeague One · England | 2–1 | League One · England |
| 2009-04-25 | GillinghamvBuryLeague Two · England | 0–0 | League Two · England |
| 2009-04-25 | Annan AthleticvBerwickLeague Two · Scotland | 1–1 | League Two · Scotland |
| 2009-04-25 | Werder BremenvBochumBundesliga · Germany | 3–2 | Bundesliga · Germany |
| 2009-04-25 | AlloavQueens ParkLeague One · Scotland | 0–0 | League One · Scotland |
| 2009-04-25 | FulhamvStokePremier League · England | 1–0 | Premier League · England |
| 2009-04-25 | Sheffield UnitedvSwanseaChampionship · England | 1–0 | Championship · England |
| 2009-04-25 | CheltenhamvCarlisleLeague One · England | 1–1 | League One · England |
| 2009-04-25 | AldershotvChesterLeague Two · England | 2–2 | League Two · England |
| 2009-04-25 | Bristol CityvSheffield WedsChampionship · England | 1–1 | Championship · England |
| 2009-04-25 | RiminivAvellinoSerie B · Italy | 2–3 | Serie B · Italy |
| 2009-04-25 | DortmundvHamburgBundesliga · Germany | 2–0 | Bundesliga · Germany |
| 2009-04-25 | MacclesfieldvBarnetLeague Two · England | 2–1 | League Two · England |
| 2009-04-25 | EibarvZaragozaLa Liga 2 · Spain | 2–3 | La Liga 2 · Spain |
| 2009-04-25 | HartlepoolvLeedsLeague One · England | 0–1 | League One · England |
| 2009-04-25 | CharleroivWaregemPro League · Belgium | 1–0 | Pro League · Belgium |
| 2009-04-25 | MontrosevCowdenbeathLeague Two · Scotland | 2–1 | League Two · Scotland |
| 2009-04-25 | PiacenzavEmpoliSerie B · Italy | 1–1 | Serie B · Italy |
| 2009-04-25 | Sp LisbonvEst AmadoraPrimeira Liga · Portugal | 2–1 | Primeira Liga · Portugal |
| 2009-04-25 | DumbartonvForfarLeague Two · Scotland | 4–0 | League Two · Scotland |
| 2009-04-25 | HuddersfieldvBrightonLeague One · England | 2–2 | League One · England |
| 2009-04-25 | CastellonvHerculesLa Liga 2 · Spain | 0–0 | La Liga 2 · Spain |
| 2009-04-25 | LutonvRochdaleLeague Two · England | 1–1 | League Two · England |
| 2009-04-25 | EvertonvMan CityPremier League · England | 1–2 | Premier League · England |
| 2009-04-25 | LivornovModenaSerie B · Italy | 1–2 | Serie B · Italy |
| 2009-04-25 | TranmerevYeovilLeague One · England | 1–1 | League One · England |
| 2009-04-25 | MalagavLa CorunaLa Liga · Spain | 1–1 | La Liga · Spain |
| 2009-04-25 | ToursvGuingampLigue 2 · France | 1–0 | Ligue 2 · France |
| 2009-04-25 | TrevisovTriestinaSerie B · Italy | 1–0 | Serie B · Italy |
| 2009-04-25 | ExetervMorecambeLeague Two · England | 2–2 | League Two · England |
| 2009-04-25 | Milton Keynes DonsvWalsallLeague One · England | 0–1 | League One · England |
| 2009-04-25 | BournemouthvGrimsbyLeague Two · England | 2–1 | League Two · England |
| 2009-04-25 | Man UnitedvTottenhamPremier League · England | 5–2 | Premier League · England |
| 2009-04-25 | BradfordvRotherhamLeague Two · England | 3–0 | League Two · England |
| 2009-04-25 | AnderlechtvDenderPro League · Belgium | 4–0 | Pro League · Belgium |
| 2009-04-25 | BergenvWesterloPro League · Belgium | 0–2 | Pro League · Belgium |
| 2009-04-25 | DoncastervCrystal PalaceChampionship · England | 2–0 | Championship · England |
| 2009-04-25 | AlbacetevCordobaLa Liga 2 · Spain | 1–1 | La Liga 2 · Spain |
| 2009-04-25 | BirminghamvPrestonChampionship · England | 1–2 | Championship · England |
| 2009-04-25 | SassuolovAscoliSerie B · Italy | 1–0 | Serie B · Italy |
| 2009-04-25 | TenerifevLas PalmasLa Liga 2 · Spain | 2–0 | La Liga 2 · Spain |
| 2009-04-24 | BrestvSedanLigue 2 · France | 2–2 | Ligue 2 · France |
| 2009-04-24 | Greuther FurthvAugsburg2. Bundesliga · Germany | 2–1 | 2. Bundesliga · Germany |
| 2009-04-24 | GerminalvClub BruggePro League · Belgium | 2–0 | Pro League · Belgium |
| 2009-04-24 | Sp BragavTrofensePrimeira Liga · Portugal | 1–0 | Primeira Liga · Portugal |
| 2009-04-24 | LeicestervScunthorpeLeague One · England | 2–2 | League One · England |
| 2009-04-24 | St PaulivFreiburg2. Bundesliga · Germany | 1–2 | 2. Bundesliga · Germany |
| 2009-04-24 | SpartavGraafschapEredivisie · Netherlands | 0–0 | Eredivisie · Netherlands |