| 2009-04-26 | KidderminstervKettering TownNational League · England | 0–1 | National League · England |
| 2009-04-26 | CambridgevAltrinchamNational League · England | 0–0 | National League · England |
| 2009-04-26 | PanathinaikosvOFISuper League · Greece | 3–1 | Super League · Greece |
| 2009-04-26 | NancyvNantesLigue 1 · France | 2–0 | Ligue 1 · France |
| 2009-04-26 | Asteras TripolisvPanioniosSuper League · Greece | 3–4 | Super League · Greece |
| 2009-04-26 | M'gladbachvBielefeldBundesliga · Germany | 1–1 | Bundesliga · Germany |
| 2009-04-26 | BursasporvDenizlisporSüper Lig · Turkey | 2–0 | Süper Lig · Turkey |
| 2009-04-26 | OberhausenvMunich 18602. Bundesliga · Germany | 1–1 | 2. Bundesliga · Germany |
| 2009-04-26 | Le Mansv MonacoLigue 1 · France | 0–1 | Ligue 1 · France |
| 2009-04-26 | LillevMarseilleLigue 1 · France | 1–2 | Ligue 1 · France |
| 2009-04-26 | XerezvSevilla BLa Liga 2 · Spain | 2–0 | La Liga 2 · Spain |
| 2009-04-26 | Eastbourne BoroughvBarrowNational League · England | 0–2 | National League · England |
| 2009-04-26 | RegginavJuventusSerie A · Italy | 2–2 | Serie A · Italy |
| 2009-04-26 | Ath BilbaovSantanderLa Liga · Spain | 2–1 | La Liga · Spain |
| 2009-04-26 | GetafevVillarrealLa Liga · Spain | 1–2 | La Liga · Spain |
| 2009-04-26 | Ath MadridvSp GijonLa Liga · Spain | 3–1 | La Liga · Spain |
| 2009-04-26 | ValladolidvOsasunaLa Liga · Spain | 0–0 | La Liga · Spain |
| 2009-04-26 | LevadeiakosvLarisaSuper League · Greece | 0–1 | Super League · Greece |
| 2009-04-26 | Le HavrevGrenobleLigue 1 · France | 0–1 | Ligue 1 · France |
| 2009-04-26 | EspanolvBetisLa Liga · Spain | 2–0 | La Liga · Spain |
| 2009-04-26 | Rushden & DvWokingNational League · England | 3–1 | National League · England |
| 2009-04-26 | Frankfurt FSVvNurnberg2. Bundesliga · Germany | 2–1 | 2. Bundesliga · Germany |
| 2009-04-26 | GenclerbirligivGaziantepsporSüper Lig · Turkey | 1–0 | Süper Lig · Turkey |
| 2009-04-26 | AlicantevVallecanoLa Liga 2 · Spain | 2–0 | La Liga 2 · Spain |
| 2009-04-26 | ErgotelisvAEKSuper League · Greece | 1–1 | Super League · Greece |
| 2009-04-26 | NavalvRio AvePrimeira Liga · Portugal | 0–1 | Primeira Liga · Portugal |
| 2009-04-26 | SevillavReal MadridLa Liga · Spain | 2–4 | La Liga · Spain |
| 2009-04-26 | OxfordvNorthwichNational League · England | 1–2 | National League · England |
| 2009-04-26 | RoeselarevGentPro League · Belgium | 1–3 | Pro League · Belgium |
| 2009-04-26 | BolognavGenoaSerie A · Italy | 2–0 | Serie A · Italy |
| 2009-04-26 | ToulousevLorientLigue 1 · France | 1–1 | Ligue 1 · France |
| 2009-04-26 | Crawley TownvHistonNational League · England | 3–3 | National League · England |
| 2009-04-26 |  Bangladeshv CambodiaAFC Challenge Cup qualification | 1–0 | AFC Challenge Cup qualification |
| 2009-04-26 | AjaxvAZ AlkmaarEredivisie · Netherlands | 1–1 | Eredivisie · Netherlands |
| 2009-04-26 | GraysvForest GreenNational League · England | 2–1 | National League · England |
| 2009-04-26 | SalamancavElcheLa Liga 2 · Spain | 1–1 | La Liga 2 · Spain |
| 2009-04-26 | CottbusvWolfsburgBundesliga · Germany | 2–0 | Bundesliga · Germany |
| 2009-04-26 | KoblenzvAachen2. Bundesliga · Germany | 0–2 | 2. Bundesliga · Germany |
| 2009-04-26 | LewesvYorkNational League · England | 1–1 | National League · England |
| 2009-04-26 | KocaelisporvAntalyasporSüper Lig · Turkey | 1–0 | Süper Lig · Turkey |
| 2009-04-26 | RecreativovMallorcaLa Liga · Spain | 2–4 | La Liga · Spain |
| 2009-04-26 | XanthivIraklisSuper League · Greece | 4–1 | Super League · Greece |
| 2009-04-26 | GroningenvWillem IIEredivisie · Netherlands | 0–0 | Eredivisie · Netherlands |
| 2009-04-26 | KaiserslauternvWehen2. Bundesliga · Germany | 1–0 | 2. Bundesliga · Germany |
| 2009-04-26 | LaziovAtalantaSerie A · Italy | 0–1 | Serie A · Italy |
| 2009-04-26 | HuescavSociedadLa Liga 2 · Spain | 1–2 | La Liga 2 · Spain |
| 2009-04-26 | KayserisporvKonyasporSüper Lig · Turkey | 1–1 | Süper Lig · Turkey |
| 2009-04-26 | EskisehirsporvBesiktasSüper Lig · Turkey | 0–2 | Süper Lig · Turkey |
| 2009-04-25 | GrossetovVicenzaSerie B · Italy | 2–1 | Serie B · Italy |
| 2009-04-25 | Port ValevWycombeLeague Two · England | 1–1 | League Two · England |