| 2010-04-24 | Queens ParkvElginLeague Two · Scotland | 0–1 | League Two · Scotland |
| 2010-04-24 | PrestonvLeicesterChampionship · England | 0–1 | Championship · England |
| 2010-04-24 | LeverkusenvHannoverBundesliga · Germany | 3–0 | Bundesliga · Germany |
| 2010-04-24 | Villarreal BvCeltaLa Liga 2 · Spain | 1–0 | La Liga 2 · Spain |
| 2010-04-24 | Real UnionvMurciaLa Liga 2 · Spain | 2–0 | La Liga 2 · Spain |
| 2010-04-24 | Werder BremenvFC KolnBundesliga · Germany | 1–0 | Bundesliga · Germany |
| 2010-04-24 | TamworthvEbbsfleetNational League · England | 3–4 | National League · England |
| 2010-04-24 | GenclerbirligivAntalyasporSüper Lig · Turkey | 0–2 | Süper Lig · Turkey |
| 2010-04-24 | GraysvForest GreenNational League · England | 2–1 | National League · England |
| 2010-04-24 | BarnetvRotherhamLeague Two · England | 0–1 | League Two · England |
| 2010-04-24 | DundeevRaith RvsChampionship · Scotland | 2–0 | Championship · Scotland |
| 2010-04-24 | BrightonvBristol RvsLeague One · England | 2–1 | League One · England |
| 2010-04-24 | SalisburyvKettering TownNational League · England | 2–0 | National League · England |
| 2010-04-24 | Man UnitedvTottenhamPremier League · England | 3–1 | Premier League · England |
| 2010-04-24 | East FifevStenhousemuirLeague One · Scotland | 1–1 | League One · Scotland |
| 2010-04-24 | Ross CountyvPartickChampionship · Scotland | 1–2 | Championship · Scotland |
| 2010-04-24 | OldhamvSouthendLeague One · England | 2–2 | League One · England |
| 2010-04-24 | PalermovMilanSerie A · Italy | 3–1 | Serie A · Italy |
| 2010-04-24 | NurnbergvDortmundBundesliga · Germany | 2–3 | Bundesliga · Germany |
| 2010-04-24 | ColchestervTranmereLeague One · England | 1–1 | League One · England |
| 2010-04-24 | NewcastlevIpswichChampionship · England | 2–2 | Championship · England |
| 2010-04-24 | BesiktasvSivassporSüper Lig · Turkey | 2–2 | Süper Lig · Turkey |
| 2010-04-24 | Bristol CityvDerbyChampionship · England | 2–1 | Championship · England |
| 2010-04-24 | PeterheadvAlloaLeague One · Scotland | 2–0 | League One · Scotland |
| 2010-04-24 | Hayes & YeadingvWrexhamNational League · England | 0–1 | National League · England |
| 2010-04-24 | StevenagevYorkNational League · England | 1–0 | National League · England |
| 2010-04-24 | SetubalvPortoPrimeira Liga · Portugal | 2–5 | Primeira Liga · Portugal |
| 2010-04-24 | CittadellavMantovaSerie B · Italy | 6–0 | Serie B · Italy |
| 2010-04-24 | ExetervCharltonLeague One · England | 1–1 | League One · England |
| 2010-04-24 | CrotonevTorinoSerie B · Italy | 1–0 | Serie B · Italy |
| 2010-04-24 | BurtonvBournemouthLeague Two · England | 0–2 | League Two · England |
| 2010-04-24 | DuisburgvPaderborn2. Bundesliga · Germany | 2–3 | 2. Bundesliga · Germany |
| 2010-04-24 | WycombevSwindonLeague One · England | 2–2 | League One · England |
| 2010-04-24 | AlbacetevHuescaLa Liga 2 · Spain | 0–0 | La Liga 2 · Spain |
| 2010-04-24 | AltrinchamvCambridgeNational League · England | 0–2 | National League · England |
| 2010-04-24 | AyrvInverness CChampionship · Scotland | 0–7 | Championship · Scotland |
| 2010-04-24 | TorquayvMacclesfieldLeague Two · England | 1–0 | League Two · England |
| 2010-04-24 | BarnsleyvQPRChampionship · England | 0–1 | Championship · England |
| 2010-04-24 | LincolnvBuryLeague Two · England | 1–0 | League Two · England |
| 2010-04-24 | DunfermlinevMortonChampionship · Scotland | 4–1 | Championship · Scotland |
| 2010-04-24 | BresciavRegginaSerie B · Italy | 0–0 | Serie B · Italy |
| 2010-04-24 | SouthamptonvCarlisleLeague One · England | 3–2 | League One · England |
| 2010-04-23 | NantesvSedanLigue 2 · France | 1–3 | Ligue 2 · France |
| 2010-04-23 | MetzvArlesLigue 2 · France | 1–2 | Ligue 2 · France |
| 2010-04-23 | Rio AvevMaritimoPrimeira Liga · Portugal | 0–0 | Primeira Liga · Portugal |
| 2010-04-23 | KaiserslauternvHansa Rostock2. Bundesliga · Germany | 0–1 | 2. Bundesliga · Germany |
| 2010-04-23 | IstresvBrestLigue 2 · France | 1–0 | Ligue 2 · France |
| 2010-04-23 | DijonvClermontLigue 2 · France | 2–3 | Ligue 2 · France |
| 2010-04-23 | CesenavEmpoliSerie B · Italy | 2–3 | Serie B · Italy |
| 2010-04-23 | KarlsruhevAhlen2. Bundesliga · Germany | 1–0 | 2. Bundesliga · Germany |