| 2021-12-26 | TranmerevBarrowLeague Two · England | 2–0 | League Two · England |
| 2021-12-18 | TranmerevLeyton OrientLeague Two · England | 1–0 | League Two · England |
| 2021-12-11 | ExetervTranmereLeague Two · England | 0–1 | League Two · England |
| 2021-12-07 | OldhamvTranmereLeague Two · England | 0–1 | League Two · England |
| 2021-11-23 | TranmerevBradfordLeague Two · England | 2–1 | League Two · England |
| 2021-11-20 | Bristol RvsvTranmereLeague Two · England | 2–2 | League Two · England |
| 2021-11-13 | TranmerevSuttonLeague Two · England | 0–1 | League Two · England |
| 2021-10-30 | MansfieldvTranmereLeague Two · England | 2–0 | League Two · England |
| 2021-10-23 | TranmerevNorthamptonLeague Two · England | 0–2 | League Two · England |
| 2021-10-19 | HarrogatevTranmereLeague Two · England | 2–2 | League Two · England |
| 2021-10-16 | CarlislevTranmereLeague Two · England | 0–1 | League Two · England |
| 2021-10-08 | TranmerevColchesterLeague Two · England | 2–0 | League Two · England |
| 2021-10-02 | TranmerevCrawley TownLeague Two · England | 2–1 | League Two · England |
| 2021-09-25 | Forest GreenvTranmereLeague Two · England | 0–0 | League Two · England |
| 2021-09-18 | TranmerevSalfordLeague Two · England | 2–0 | League Two · England |
| 2021-09-11 | RochdalevTranmereLeague Two · England | 1–0 | League Two · England |
| 2021-09-04 | TranmerevHartlepoolLeague Two · England | 1–0 | League Two · England |
| 2021-08-28 | ScunthorpevTranmereLeague Two · England | 1–0 | League Two · England |
| 2021-08-21 | TranmerevNewport CountyLeague Two · England | 0–1 | League Two · England |
| 2021-08-17 | SwindonvTranmereLeague Two · England | 0–0 | League Two · England |
| 2021-08-14 | Port ValevTranmereLeague Two · England | 0–0 | League Two · England |
| 2021-08-07 | TranmerevWalsallLeague Two · England | 1–0 | League Two · England |
| 2021-05-08 | TranmerevColchesterLeague Two · England | 0–0 | League Two · England |
| 2021-05-01 | Forest GreenvTranmereLeague Two · England | 2–1 | League Two · England |
| 2021-04-24 | TranmerevBarrowLeague Two · England | 1–0 | League Two · England |
| 2021-04-20 | BradfordvTranmereLeague Two · England | 0–1 | League Two · England |
| 2021-04-17 | TranmerevSalfordLeague Two · England | 0–0 | League Two · England |
| 2021-04-13 | WalsallvTranmereLeague Two · England | 1–0 | League Two · England |
| 2021-04-10 | ScunthorpevTranmereLeague Two · England | 0–0 | League Two · England |
| 2021-04-05 | TranmerevCambridgeLeague Two · England | 1–1 | League Two · England |
| 2021-04-02 | CheltenhamvTranmereLeague Two · England | 4–0 | League Two · England |
| 2021-03-27 | TranmerevMansfieldLeague Two · England | 1–1 | League Two · England |
| 2021-03-23 | ColchestervTranmereLeague Two · England | 2–2 | League Two · England |
| 2021-03-20 | TranmerevExeterLeague Two · England | 2–1 | League Two · England |
| 2021-03-17 | GrimsbyvTranmereLeague Two · England | 0–0 | League Two · England |
| 2021-03-09 | SouthendvTranmereLeague Two · England | 0–2 | League Two · England |
| 2021-03-06 | TranmerevCrawley TownLeague Two · England | 0–1 | League Two · England |
| 2021-03-02 | TranmerevNewport CountyLeague Two · England | 1–0 | League Two · England |
| 2021-02-27 | Leyton OrientvTranmereLeague Two · England | 1–3 | League Two · England |
| 2021-02-23 | CarlislevTranmereLeague Two · England | 2–3 | League Two · England |
| 2021-02-20 | TranmerevOldhamLeague Two · England | 2–2 | League Two · England |
| 2021-02-09 | TranmerevStevenageLeague Two · England | 0–1 | League Two · England |
| 2021-02-06 | TranmerevPort ValeLeague Two · England | 3–1 | League Two · England |
| 2021-01-30 | MorecambevTranmereLeague Two · England | 0–1 | League Two · England |
| 2021-01-26 | TranmerevHarrogateLeague Two · England | 3–2 | League Two · England |
| 2021-01-23 | TranmerevBoltonLeague Two · England | 2–1 | League Two · England |
| 2021-01-19 | TranmerevForest GreenLeague Two · England | 3–2 | League Two · England |
| 2021-01-16 | StevenagevTranmereLeague Two · England | 0–0 | League Two · England |